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| Registros recuperados: 273 | |
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| Batista, Frederico; Leitao, Alexandra; Huvet, Arnaud; Lapegue, Sylvie; Heurtebise, Serge; Boudry, Pierre. |
| The taxonomic status of the Portuguese oyster Crassostrea angulata and the Pacific oyster Crassostrea gigas has often been a matter of controversy. Based on larval shell morphology, experimental hybridisation and electrophoretic studies of enzyme polymorphism several authors have considered these two species as being synonymous. During the recent years, several genetic studies based on mitochondrial DNA and microsatellites data have provided evidences that the two taxa are genetically distinct although closely related. Furthermore, karyotype analyses have also highlighted the close genetic similarity of these taxa in comparison with other cupped oyster species. However, a comparative analysis of restriction enzymes ideograms revealed differences between... |
| Tipo: Text |
Palavras-chave: Geographical origin; Phylogenetic; Genetic; Taxonomic status; Crassostrea angulata; Oyster. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3433.pdf |
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| Boudry, Pierre; Collet, Bertrand; Kotoulas, Georgios; Magoulas, Antonio; Hervouet, Veronique; Bonhomme, Francois; Gerard, Andre. |
| Highly polymorphic genetic markers can be useful tools for the analysis of parental contributions. The present paper reports 3 experiments using microsatellite markers to analyse parental contributions in controlled in vitro factorial crosses of the cupped oyster Crassostrea gigas. Parentage analysis was eased by the large polymorphism observed at the 3 loci studied. In a cross between 20 males and 20 females, a quarter of the progeny could be assigned to a given parent using a single microsatellite locus showing 28 alleles. In 3 crosses between 5 males and females, all the progeny could be assigned to a family by using 3 loci. In all cases, the combined genotyping at 2 loci allowed parentage to be detennined unambiguously. Despite the balanced gametic... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Microsatellites; Parental contributions; Crassostrea gigas; Microsatellites; Contributions parentales. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/acte-2739.pdf |
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| Lapegue, Sylvie; Heurtebise, Serge; Harrang, Estelle; Morga, Benjamin; Flahauw, Emilie; Sauvage, Christopher; Boudry, Pierre. |
| During the last five years, a great effort has been made to develop genomic tools in oyster species all over the world. In Europe, the Pacific cupped oyster, Crassostrea gigas, and the European flat oyster, Ostrea edulis received a special attention as they are the key species for local aquaculture. In particular, the Pacific oyster is a marine bivalve of major economical and ecological importance worldwide. Better knowledge of its physiology and its genetics contributes to optimize its aquaculture production using selective breeding or to understand its invasive behavior in some coastal areas. Current international developments of genomic resources include EST databases, BAC libraries, genome mapping and sequencing. The Genoscope (Evry, France) and the... |
| Tipo: Text |
Palavras-chave: Oysters; Crassostrea gigas; Ostrea edulis; Resistance pathogens; QTLs; Single Nucleotide Polymorphism. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00015/12584/9454.pdf |
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| Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Heurtebise, Serge; Garcia, P; Thiriot Quievreux, C; Boudry, Pierre. |
| Three species of man.-rove oysters, Crassostrea rhizophorae, C. brasiliana, and C. gasar, have been described along the Atlantic shores of South America and Africa. Because the distribution of these molluscs is of great biological and commercial interest, their taxonomy and distribution deserve further clarification. Therefore, 15 populations were sampled from both continents. Their 16S mitochondrial polymorphism was studied by sequencing and PCR-RFLP analysis. Two haplotypes were identified. Haplotype a was the only one observed in Africa, but it was also observed in South America together with haplotype b. Because C. gasar is the only mangrove oyster identified on the west coast of Africa, haplotype a was attributed to this species, which has thus been... |
| Tipo: Text |
Palavras-chave: Mangrove oyster; Crassostrea gasar; Karyotype; Phylogeography; 16S mitochondrial DNA. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-2790.pdf |
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| Harney, Ewan; Lachambre, Sebastien; Roussel, Sabine; Huchette, Sylvain; Enez, Florian; Morvezen, Romain; Haffray, Pierrick; Boudry, Pierre. |
| Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by... |
| Tipo: Text |
Palavras-chave: Abalone; Parentage assignment; Pedigree; Selective breeding; Transcriptome; SNP. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00429/54061/55396.pdf |
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| Dheilly, Nolwenn M.; Jouaux, Aude; Boudry, Pierre; Favrel, Pascal; Lelong, Christophe. |
| Background: Triploidy can occur in many animal species but is often lethal. Among invertebrates, amphibians and fishes, triploids are viable although often sterile or infertile. Most triploids of the Pacific oyster Crassostrea gigas are almost sterile (named "3n beta'') yet a low but significant proportion show an advanced gametogenesis (named "3n alpha''). These oysters thus constitute an interesting model to study the effect of triploidy on germ cell development. We used microarrays to compare the gonad transcriptomes of diploid 2n and the abovementioned triploid 3n beta and 3n alpha male and female oysters throughout gametogenesis. Results: All triploids displayed an upregulation of genes related to DNA repair and apoptosis and a downregulation of genes... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00239/34992/33538.pdf |
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| Boudry, Pierre; Collet, Bertrand; Kotoulas, G.; Hervouet, Veronique; Cornette, Florence; Bonhomme, Francois; Gerard, Andre. |
| Numerous aquacultural species show very high fertilities. As a consequence, it is possible to produce, in hatcheries, a large amount of sibs from a small amount of parents. The sibs' genetic variability is, of course, directly linked to the number of parents involved, but it may be largely lowered by imbalanced parental contributions. Polymorphic genetic markers are very handy tools in the study of these phenomena in cultivated populations (Harris et al. 1991; Herbinger et al., 1996a; Garcia de Leon et al. 1998) or in natural populations (Ferguson et al, 1995; Kellog et al., 1995; Colbourne et al., 1996; Herbinger et al., 1996b). This document is a presentation of the results of a number of experiments, realised in the scope of the European program... |
| Tipo: Text |
Palavras-chave: Microsatellites; Contributions parentales; Crassostrea gigas; Huîtres. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/acte-3452.pdf |
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| Huvet, Arnaud; Boudry, Pierre; Ohresser, M; Delsert, Claude; Bonhomme, F. |
| Source/description : Genomic DNA was extracted from a whole Pacific oyster (C. gigas) after grinding in liquid nitrogen. Purified DNA was then digested by a mix of 3 restriction enzymes (AluI, RsaI, HaeIII). DNA fragments ranging from 250 to 500 bp were size-selected by agarose gel electrophoresis and ligated into the dephosphorylated blunt-ended SmaI-linearised pBKS2 plasmid (Stratagene Cloning System, CA). White colonies (11,500) were screened by hybridisation with double-stranded DNA probes containing dinucleotide repeats. Poly(dAdC), poly(dAdG), poly(dAdT) (Pharmacia Biotech, Sweden) were mixed at equimolar ratio and radiolabelled by [-32P] alpha dATP and dCTP using a random priming labelling kit (Life Technologies, Germany). Filter... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Oyster; Microsatellites. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-703.pdf |
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| Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie. |
| In order to document further the phenomena of variance in reproductive success in natural populations of the European flat oyster Ostrea Midis, two complementary studies based on natural and experimental populations were conducted. The first part of this work was focused on paternity analyses using a set of four microsatellite markers for larvae collected from 13 brooding females sampled in Quiberon Bay (Brittany, France). The number of individuals contributing as the male parent to each progeny assay was highly variable, ranging from 2 to more than 40. Moreover, paternal contributions showed a much skewed distribution, with some males contributing to 50-100% of the progeny assay. The second part of this work consisted of the analysis of six successive... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00012/12329/9222.pdf |
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| Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie. |
| he European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. Previous studies of allozymes, microsat-ellites and mitochondrial differentiation over the whole range concluded that a significant divergence existed between Mediterranean and Atlantic populations, together with an isolation-by-distance pattern. However, the average mitochondrial haplotypic diversity displayed a high among populations variance, reflecting smaller effective population size in some locations. Additionally, a ten-fold quantitative difference was observed in the same study in Fst between the mitochondrial and the nuclear genomes,... |
| Tipo: Text |
Palavras-chave: Genotypes; Genetic diversity; Breeding success; Allozymes; Population genetics; Parentage analyses; Natural population; Ostrea edulis; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3334.pdf |
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| Wenne, Roman; Boudry, Pierre; Hemmer-hansen, Jakob; Lubieniecki, Krzysztof P.; Was, Anna; Kause, Antti. |
| The development and application of genomics has been facilitated in a number of fields by the availability of new methodologies and tools, such as high throughput DNA sequencing and complementary DNA (cDNA) microarrays. Genomic tools are already used in research on commercially important fish and shellfish species. Thousands of expressed sequence tags (EST) are now available for some of these species, and the sequencing of complete genomes of tilapia, cod, salmonids, flatfishes, sea bass and Pacific oyster has been proposed. Microarray technology through simultaneous analysis of the expression of thousands of genes allows the identification of candidate genes involved in the function of multiple physiological, morphological and behavioural traits of... |
| Tipo: Text |
Palavras-chave: Oyster; Fish; Quantitative trait loci; Mariculture; Fisheries; Genetics; Genomics. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2927.pdf |
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| Registros recuperados: 273 | |
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